Their origin remains unknown, as well as the origin of their "cruisers".
StrategyMutation Agents always appear during gated hordes. When the player deals with all waves, the Agent shows itself along with one final wave of Darkspore Mutants. Mutation Agents will continously pump out clouds of E-DNA, dealing DPS damage, when the heroes venture in the cloud's range. In addition, they can channel a stream of superpowered E-DNA to a Darkspore, turning it into an Elite, making the fight even worse. In addition, Mutation Agents are orbular, making Skar's Opportunism utterly useless.
The best strategy to deal with Mutation Agents is to kill all accompanying Darkspore as soon as possible. An alternative strategy, is to immediately focus on the Mutation Agent. If there even will be some Elites, that will be spawned, deal with the other Darkspore first to prevent more Elites from coming onto the battlefield. When the Mutation Agent is all alone, use a Ranged character, most likely a Tempest, to keep yourself out of the E-DNA cloud. After that all you need to do, is shoot and watch as the Mutation Agent loses health. When all Darkspore including the Agent will be killed, the gates will perish and retreat to the ground.
- Mutation Agents seem to be the official trademark for Darkspore. Which would replace a certain character from the status of the "Darkspore Mascot".